Fig. 5
From: IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma
IL-25 and IL-33 prime eotaxin-induced basophil shape change. Purified peripheral blood basophils from 8 allergic donors were suspended in RPMI-C and incubated with/without IL-25 and IL-33 (10 ng/mL) to assess (a) CCR3 expression and (b, c) shape change was measured via change in FSC post-stimulation with eotaxin (5 ng/mL). The effect of neutralizing antibodies for (d) IL-17RB and (e) ST2 (light grey bars) on shape change was also assessed. Data are expressed as mean ± SEM. ‡ Significantly different from eotaxin, *significantly different from PBS negative control, †significantly different from isotype control; P < 0.05 (N = 8)