Fig. 6

Effect of equivalent concentrations of budesonide (BUD) and fluticasone propionate (FP) on cigarette smoke extract (CSE) and/or poly-(I:C)-induced barrier dysfunction in 16HBE cells. 16HBE cells were seeded in duplicates, grown to confluence, serum-deprived overnight, pre-treated with or without 16 nM BUD or 10 nM FP for 2 hours and exposed to vehicle (control), poly-(I:C) (a) or 7.5 % CSE plus poly-(I:C) (b). Resistance was measured at 400 Hz using ECIS. Resistance levels were normalized to the levels prior to the addition of poly-(I:C) and mean ± SEM levels (n = 3−4) are shown. In the upper panels, p < 0.01 for control versus poly-(I:C), p < 0.05 for poly-(I:C) versus poly-(I:C) + BUD and poly-(I:C) + FP (a), p = 0.05 for control versus CSE + poly-(I:C), p < 0.05 for CSE + poly-(I:C) versus CSE + poly-(I:C) + BUD and p < 0.001 for CSE + poly-(I:C) + BUD versus CSE + poly-(I:C) + FP (b), as analyzed by repeated measures ANOVA. The area-under-the-curve (AUC) was calculated and mean ± SEM levels (n = 3−4) are shown. * = p < 0.05 and ** = p < 0.01 between the indicated groups (repeated measures ANOVA with Bonferroni’s post-hoc test)