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Fig. 3 | Respiratory Research

Fig. 3

From: TNF-α and IL-1β-activated human mesenchymal stromal cells increase airway epithelial wound healing in vitro via activation of the epidermal growth factor receptor

Fig. 3

MSC-CMSTIM increases ERK1/2 phosphorylation. NCI-H292 cells were incubated with MSC-CMSTIM, DMEMSTIM, DMEMCTRL or TGF-α 20 ng/ml (pos ctrl). ERK1/2 phosphorylation and total ERK was determined in cell lysates using Western blot. a: After 15 min incubation time MSC-CMSTIM increased ERK1/2 phosphorylation compared to its control. b: Densitometry for Fig. 3a. Error bars represent SEM, n = 6; (*) p < 0.05. c: Time course experiment, demonstrating that the effect of MSC-CMSTIM was most prominent up to 30 min, but could still be observed up to 6 h. DMEMCTRL was obtained at 15 min. d: Densitometry for Fig. 3c. Error bars represent SEM, n = 4-5; (*) p < 0.05

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