Fig. 5

YKL-40 protein secretion and mRNA expression is inhibited by the demethylating agent 5AZA. IL-10 and IL-12p40 secretion after stimulation with 5-AZA (concentration 0.1, 1 and 10 μM) (a and b, respectively). YKL-40 protein secretion and mRNA expression in MΦ1 and MΦ2 after culturing with 5-AZA (concentration 0.1, 0.3 and 1nM) (c and d, respectively). Differentiated cells are stimulated with 100 ng/ml LPS for 24 h. Data represent mean and SEM (n = 3 donors). * p ≤ 0.05, ** p < 0.01 or *** p < 0.001 compared to MΦ2 with corresponding stimulus. # p ≤ 0.05, ##: p < 0.01 or ### p < 0.001 compared to corresponding LPS stimulated cells