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Fig. 2 | Respiratory Research

Fig. 2

From: Regulation of YKL-40 expression by corticosteroids: effect on pro-inflammatory macrophages in vitro and its modulation in COPD in vivo

Fig. 2

MΦ1 produce more YKL-40 compared to MΦ2. a: YKL-40 secretion measured by ELISA (left panel, 6 donors) and mRNA levels (normalized expression, right panel, 5 donors) in MΦ1 and MΦ2 after medium and LPS stimulation for 24 h. * p < 0.05 or *** p < 0.001 between MΦ1 and MΦ2. b: Flow cytometry analysis of MΦ1 and MΦ2 with and without anti-YKL-40 and secondary antibody. Left panel: MΦ1 with secondary antibody (purple) and MΦ1 with anti-YKL-40 and secondary antibody (black); middle panel: MΦ2 with secondary antibody alone (purple) and MΦ2 with anti-YKL-40 and secondary antibody (black); right panel: MΦ1 and anti-YKL-40 with secondary antibody (red) and MΦ2 and anti-YKL-40 with secondary antibody (blue). c: YKL-40 secretion in MΦ1 and MΦ2 after 24 and 48 h of stimulation with medium, LPS, TNFα and oncostatin M (OSM). Data represent mean and SEM of 4 and 3 donors (24 and 48 h, respectively). *: p < 0.001 between MΦ1 and MΦ2 for corresponding stimulus and time. $: p < 0.001 between corresponding stimulus at different time points. d: Immunofluorescence staining on sputum cytospin of a COPD patient (blue: DAPI; red: YKL-40; green: CD68)

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