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Fig. 3 | Respiratory Research

Fig. 3

From: Overexpression or absence of calretinin in mouse primary mesothelial cells inversely affects proliferation and cell migration

Fig. 3

a Real-time growth curves of WT prMC (control), cells transfected with a SV40 plasmid (SV40) or a CR expression plasmid (LV-CR). b MTT signals at 96 h (normalized to WT cells) are increased in cells expressing SV40 TAg/tag. c Growth curves for CR−/− prMC subjected to the same treatment as in (a). d Normalized MTT signals in CR−/− cells; all experimental details are the same as in (b) (n = 3, one representative growth curve and MTT experiment is shown). Western Blot analysis in (e) show a strong upregulation of CR and SV40 large T Antigen (TAg) in the corresponding cell cultures. α-Mesothelin is used as marker for mesothelial cells and α-actin serves as a loading control. f Immunohistochemistry for CR (red) in CR-overexpressing WT cells and cytokeratin (green), cell nuclei are stained with DAPI (blue) (***p < 0.0005)

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Respiratory Research

ISSN: 1465-993X

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