Prostaglandin E2 stimulates normal bronchial epithelial cell growth through induction of c-Jun and PDK1, a kinase implicated in oncogenesis

Table 1 Reverse transcription and real time PCR primer

	Stage	Temp	Time
Reverse transcription	hold	37 °C	120 min
Reverse transcription	hold	25 °C	10 min
PCR	hold	50 °C	2 min
	denature	95 °C	15 s
	anneal/extend	60 °C	1 min
	hold cycle (40 cycles)	95 °C	10 min
Primer	Sense 5′ AGATGAGTGACCGAGGAG
Primer	Antisense 3′ TATACACTGGGAGTCTTTCT

ISSN: 1465-993X