Fig. 3

Western analysis confirming phosphorylation and nuclear traslocation of STAT3 protein following stimulation of fibroblasts with IL-21, IL-22 and IL-23 cytokines. Primary human lung fibroblasts were stimulated with IL-21, 22 and 23 cytokines alone or in combination for 15 min, cells lysed, fractionated, and proteins resolved using western blotting. a Western blot of cell lysates probed with anti-p-STAT3 and anti-STAT3 (b) Densitometry of p-STAT3 immunoreactive bands relative to total STAT3. IL-6 was used as positive control. c Western blot of cytoplasmic [C] and nuclear [N] fractions probed with anti-STAT3, anti-Lamin B (nuclear marker), and anti-β-actin antibodies (d) Densitometry of nuclear STAT3 immunoreactive bands relative to cytoplasmic STAT3. (n = 5). Comparison is always between cells treated with cytokines and non-treated cells. Data is expressed as means ± SE. * p ≤0.05. NS non-stimulated