Fig. 5From: Investigating NF-κB signaling in lung fibroblasts in 2D and 3D culture systemsImmunostaining of TNF receptor I expression of lung fibroblasts in 2D and 3D culture systems. TNF-α receptor I on fibroblast surface was immunostained with Alexa Fluor® 488 secondary antibody (green) in both 2D (a–b) and 3D (c–d) culture systems and nuclei were labeled with DAPI (blue). (Bar =50 μm, n = 3)Back to article page