Fig. 3
From: Investigating NF-κB signaling in lung fibroblasts in 2D and 3D culture systems
TNF-α induced NF-κB Activation in MRC5 lung fibroblasts in 3D culture system. Translocation of NF-κB/p65 was detected in MRC5 fibroblasts grown in 3D culture system after exposure to TNF-α (2 ng/mL) for 1 h by immunostaining (green color). Nuclei of lung fibroblasts were localized by DAPI (blue) (a and d). a–c represents cytoplasmic localization of NF-κB/p65 without TNF-α–treatment whereas d–f represents nuclear localization of NF-κB/p65 in MRC5 after TNF-α treatment for 1 h. The nuclear localization of NF-κB/p65 was overlaid by DAPI nuclear staining (f). (Bar =50 μm, n = 3)