Fig. 5

a Representative laser confocal images of HDAC2 staining (red) in FACS-sorted CD28null (right) and CD28+ T cells (left). Blue was DAPI counterstaining. Scale bar = 8um. The bar graph depicts results of quantitative analysis by ImageJ. Experiments were repeated 3 times, showing similar results. *** p < 0.05. b Representative Western Blot of equal numbers of sorted CD28+ and CD28null T cells, stained for HDAC2 expression. There was a decrease in the 55kD band corresponding to the HDAC2 in CD28null T cells compared with CD28+ T cells. Bar graph showing HDAC2 expression relative to β-actin from CD28 null (CD28-) and CD28+ T cells (mean ± sem from 3 experiments)