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Fig. 5 | Respiratory Research

Fig. 5

From: Down-regulation of USP13 mediates phenotype transformation of fibroblasts in idiopathic pulmonary fibrosis

Fig. 5

The PTEN protein level in UPS13-deficient cells. a PTEN protein and mRNA levels in primary IPF fibroblasts. Relative intensity analysis of PTEN protein expression levels in primary IPF fibroblast lines (n = 5) and primary normal fibroblast lines (n = 3) were assessed by the fold-change of target protein/β-actin with standardization by setting the “normal control #1” as 1. PTEN mRNA expression was measured by real-time RT-PCR, and the fold-change was calculated by using the 2-∆∆CT method. b, c MRC-5 cells were transfected with USP13 siRNA (USP13si) or NC siRNA (NCsi) for 48 h and PTEN protein (b) and mRNA (c) levels were measured by western blot and real-time RT-PCR. d MRC-5 cells were transfected with PTEN siRNA (PTENsi) or NCsi for 48 h, and USP13 protein expression was assessed by western blot. β-actin was used as a control in both the western blot and real-time RT-PCR assays. Error bars indicate the mean ± SEM. *p <0.05, **p <0.01

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