Fig. 5

Hypoxia markers of tumor tissues of non-small cell lung cancer (NSCLC). a: Immunohistochemical (IHC) stain of tumor tissues shows higher hypoxia inducible factor 1 α (HIF-1α) expression than normal lung tissue. Red arrow, HIF-1α expression in tumor cell nucleus (magnification x200). C, cancer; N, normal lung. b: HIF-1α staining scored by a semiquantitative grading system based on tumor cells showing completely dark stained nuclei: 0, none; 1, less than 10 % positivity; 2, 10 % or greater positivity. Tissues from ten patients were used for analysis. c: Western blots show higher expression of HIF-1α in tumor tissues of NCSLC. Tumor tissues from patients after treatment or with chemonaïve tumors showing HIF-1α expression compared with normal lung tissue. N, normal; C, cancer. d: Intensity of Western blots measured using Image J. Tissues from ten patients were used. Data are mean ± SEM, *p < 0.05. e. Carbonic anhydrase IX (CA IX) expression by IHC. Five of ten tumor tissues showed positive expression, but no normal tissue showed positive expression (p < 0.05). Red arrow: CA IX expression (magnification x200). C, cancer; N, normal lung. f. Glucose transporter 1 (GLUT1)) expression by IHC. Eight of ten tumor tissues showed positive expression, but no normal tissue showed positive expression (p < 0.05). Red arrow: GLUT1 expression (magnification x200). C, cancer; N, normal lung