Fig. 2

S100A4 localization and mRNA expression in intrapulmonary vessels in mouse lungs after smoke-exposure. Immunohistochemical staining of a S100A4, b α smooth muscle actin in serial section from mouse lungs, c negative control. Bars represent 50 μm. d real-time PCR analysis of S100A4 from laser-microdissected arteries after three and eight months of smoke exposure compared to control animals. 3 m-three months, 8 m-eight months, SE-smoke exposure. The two p-values indicate the results from the linear hypothesis about difference between SE and controls at eight months tested within a 2-factorial model (p = 0.123) and the result from testing the difference between SE at 8 month against the pooled controls (p = 0.023)