Fig. 6

i.n. IFNγ reduces apoptosis in neonatal lungs at 8 dpi. Balb/c mice (5–7 days old) were infected with RSV L19 and treated with i.n. IFNγ (16 or 60 ng/g) or PBS on 1, 3, and 5 dpi. At 8 dpi right lungs were harvested, fixed with formalin and TUNEL stained. Two individuals blinded to treatment groups quantified TUNEL positive cells on each slide for each group. (A1-A5) Represents a single lung section from each animal in group A (RSV-/IFNγ-); Group B (RSV+/IFNγ-); Group C (RSV+ IFNγ 16 ng/g); and Group D (RSV+ IFNγ 60 ng/g). Images were captured at 40X magnification and the average number of apoptotic cells per lung section were quantified and graphed (E); there were 5 mice per group. * indicates a significant difference compared to RSV+ using ANOVA with a Tukey post-test; p < 0.5