Table 1 Primers and probes designed for detection of CGRP and substance P
From: The lung response to ozone is determined by age and is partially dependent on toll-Like receptor 4
CGRP | |
|---|---|
Accession number | AF330212 |
Forward primer (position 302) | 5’-AGGAGGCTGAGGGCTCTAGTG-3’ |
Reverse primer (position 358) | 5’- CAGCCGATGGGTCACACA-3’ |
Internal probe (FAM-labeled) | 5’-CTGCTCAGAAGAGATCCTGCAACACTGC-3’ |
Substance P | Â |
Accession number | NM_009311 |
Forward primer (position 212) | 5’-GACCAGATCAAGGAGGCAATG-3’ |
Reverse primer (position 260) | 5’-GGGTCTTCGGGCGATTCT-3’ |
Internal probe (TET-labeled) | 5’-CGGAGCCCTTTGAGCATCTTCTGCA-3’ |