Fig. 7

Differential expression of BARD1 epitopes in human lung fibrosis. a IHC of tissues of NSIP patients performed with anti-BARD1 N19 (a-c) and BL (d-f) antibodies. Adjacent tissue sections were also stained for α-SMA (g-i). Myofibroblast are preferentially stained by BL antibody and co-localized with α-SMA staining (arrows) in foci of loose proliferation of interstitial smooth muscle cells, as interstitial smooth muscle cells may be seen in the fibrosis pattern of NSIP. b IHC of tissues from IPF/UIP patients stained with anti-BARD1 N19 (a-c) and BL (d-f), and α-SMA (g-i) antibodies. Thickening interstitial areas stained strongly with N19 (arrow heads) (b, c). Regions that stained for α-SMA also were preferentially stained by BL (arrows) (e, f, h, i), suggesting co-expression of BARD1β and α-SMA in fibroblastic foci