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Fig. 3 | Respiratory Research

Fig. 3

From: BARD1 mediates TGF-β signaling in pulmonary fibrosis

Fig. 3

Differential effect of FL BARD1 and BARD1β on cell proliferation and apoptosis. a-b A549 cells were transfected with FL BARD1 and BARD1β expressing plasmids and cell extracts were prepared after 48 h. a Western Blots probed with anti-BARD1 (BL) and fibronectin. b RT-PCR analysis performed with primers for amplification of full length (FL) BARD1 (exon 1 to exon 11) or isoform BARD1β using primers from exon 1/4 junction and exon 11. c A549 and (d) L929 cell proliferation of control transformed cells (pcDNA), and cells expressing exogenous FL BARD1 or BARD1β was monitored over 3 days. BARD1β leads to increased proliferation, as described before. e Annexin V and PI staining of (e, f) A549 and (e, g) L929 24 h after transfection with FL BARD1, BARD1β or pcDNA control expressing plasmids. FL BARD1 induced cell apoptosis. Each bar represents mean + SD (n ≥2 experiments); two-tailed Student’s T-Test *p < 0.05, BARD1 versus control

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