Fig. 1

TGF-β modulates BARD1 expression. Human lung fibroblasts (CCD-19Lu), bronchial epithelial cells (16HBE, NuLi-1), alveolar basal epithelial cells (A549), and mouse fibroblasts (L929) were cultured in absence (NT) or presence of TGF-β1 (10 ng/ml), and cell extracts were prepared after 24, and 48 h of treatment. a Western Blots of CCD-19Lu, 16HBE and NuLi-1 cell extracts prepared at 24 and 48 h of TGF-β treatment, probed with anti-BARD1 BL (mapping exon 4 of BARD1) antibody recognizing FL BARD1 and BARD1β. b CCD19-Lu and 16HBE and (c) A549 and L929 Western Blots were quantified by measuring spot density using AlphaEaseFc software. Each bar represents mean + SD (n ≥2 experiments); two-tailed Student’s T-Test *p < 0.05, TGF-β1 versus non-treated. d A549 culture in presence of SB-431542 1 h prior TGF-β1 treatment. Cell extracts were prepared after 48 h of TGF-β1/SB-431542 treatment and probed with anti-BARD1 BL, anti-Smad2/3, anti-pSmad antibodies