Fig. 7

Effects of CLP-sepsis, Q-VD treatment (caspase inhibition), and NADPH oxidase deficiency or inhibition on pulmonary microvascular polymorphonuclear leukocyte (PMN) sequestration in murine sepsis in vivo. In septic WT mice, pulmonary microvascular/PMVEC dysfunction is associated with dramatic increases in pulmonary microvascular PMN sequestration, as assessed by rhodamine-6-G (Rh+) labeling and visualization by fluorescence IVVM. This septic pulmonary microvascular / PMVEC dysfunction and resulting increased PMN sequestration were markedly attenuated following caspase inhibition (Q-VD treatment in CLP-WT mice), in septic gp91 ^phox−/− and p47 ^phox−/− mice, and following NADPH oxidase inhibition (apocynin treatment in CLP-WT mice). n = 2-3 (Sham) and n = 3-4 (CLP). * p < 0.05, CLP vs. respective sham group; #, p < 0.05 vs. WT CLP