Fig. 4
From: Impaired survival of regulatory T cells in pulmonary sarcoidosis
Impaired survival of sarcoidosis Tregs. Isolated Tregs (purity >97 %) were cultured with recombinant human IL-2. a Percentage alive Tregs at 72 hours of culture. Horizontal line indicates the median. Significance was determined using a Mann–Whitney U test, **p < 0.01. b Representative flow cytometry analysis of an HC and SRC patient after 12 hours co-culture with autologous Th cells to determine Treg survival and apoptosis. c Survival (above) and apoptosis (below) graph of Tregs cultured for 48 hours with autologous Th cells. Dots indicate mean +/− SEM of 3 HCs and 3 SRC patients. One representative experiment is shown of 3 independent experiments. HC healthy control, SRC sarcoidosis