Fig. 9

Dexamethasone (Dex) inhibits tumour necrosis factor (TNF)-α-induced activation of nuclear factor (NF)-κB in human pulmonary arterial smooth muscle cells (PASMC). PASMC from 3 healthy human subjects were stimulated with TNF-α (0–10 ng/ml) in the presence and absence of dexamethasone (Dex, 10⁻⁷ M) for 1 h. NF-κB p65 DNA binding in nuclear extracts was measured by TransAm assay. Data are reported as the mean ± SEM. *p < 0.05, ***p < 0.001 using Kruskal Wallis and Dunn’s post-test analysis