Fig. 8

IKK-2 inhibition reduces interleukin (IL)-6 and CXCL8 release from human pulmonary arterial smooth muscle cells (PASMC). Human PASMC were plated at 5000/well in 96-well plates; starved overnight in FCS-free media and treated with tumour necrosis factor (TNF)-α (10 ng/ml) with and without the IKK-2 inhibitor AS602868 (0–3 μM) for 24 h. IL-6 (a) and CXCL8 (b) release into the supernatants was measured by ELISA. MTT assays were also performed to assess cell viability (c). Results represent mean ± SEM for n = 4, compared using 1-way ANOVA and Bonferroni’s post-test. *p < 0.05, ***p < 0.001