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Fig. 4 | Respiratory Research

Fig. 4

From: Bidirectional role of IL-6 signal in pathogenesis of lung fibrosis

Fig. 4

Endogenous IL-6 modulates BLM-sensitive cytokine production in type 2 pneumocytes. a Typical expression profile of cytokines. Primary cultured type 2 pneumocytes from mice were treated with BLM in the presence or absence of mouse IL-6-neutralizing antibody, and the supernatants were subjected to Mouse Cytokine Antibody Array C1. Application of mouse IL-6-neutralizing antibody and control IgG to the cells was performed 30 min prior to PBS or BLM treatment. The 12 molecules induced by BLM (BLM + control IgG) are numbered and indicated with the correct location in the membrane map, compared with the unstimulated control (PBS + control IgG). Among the 12 proteins, IL-6-neutralizing antibody (BLM + anti-IL-6)-induced and -reduced molecules are indicated by squares with a double-line and a single-line, respectively. IL-6-neutralizing antibody-insensitive molecules are indicated by a broken-line. We confirmed that the array profile of unstimulated control (PBS + control IgG) showed no difference from that of PBS alone. b Changes in expression of IL-6-neutralizing antibody-sensitive molecules observed in WB array analyzed by densitometer. Using a densitometer, each signal was normalized to the positive internal controls included in the array membrane and expressed in arbitrary units. Average signal of the positive internal controls is expressed as 100 arbitrary units. Data are shown as mean ± S.E.M. of three independent experiments. *P < 0.05, significantly different from value of BLM + control IgG group (ANOVA followed by Tukey’s test)

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