Fig. 3
From: Bidirectional role of IL-6 signal in pathogenesis of lung fibrosis
Endogenous IL-6 suppresses BLM-induced apoptotic signal in alveolar epithelial cells. a Effect of human IL-6-neutralizing antibody, a PI3K inhibitor LY294002, and a Stat3 inhibitor S3I-201 on BLM-induced caspase 8 activation in A549 cells. Lysates (20 μg) from cells treated with or without BLM were subjected to WB analysis for detection of cleaved caspase 8. Application of human IL-6-neutralizing antibody, control IgG, LY294002 and S3I-201 to the cells was performed 30 min prior to BLM treatment. The same lysates were subjected to WB analysis to determine the amount of β-actin as an internal control. b Exogenous IL-6 inhibited BLM-induced caspase 8 activation in A549 cells in a dose-dependent manner. The amount of β-actin of each lane was determined as an internal control. c Effect of mouse IL-6-neutralizing antibody, LY294002, and S3I-201 on BLM-induced caspase 3 activation in primary cultured SP-C+ cells. The basic procedure was the same as that for (a)