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Fig. 3 | Respiratory Research

Fig. 3

From: Plk1 regulates MEK1/2 and proliferation in airway smooth muscle cells

Fig. 3

Plk1 catalyzes MEK phosphorylation in vitro. Purified active Plk1 (40 ng) and 2 μg purified unactive MEK1 were placed in kinase buffer. MEK phosphorylation was determined by immunoblot analysis 30 min after the initiation of the reaction. a Representative immunoblots illustrates Plk1-mediated MEK phosphorylation. b MEK phosphorylation catalyzed by Plk1 was normalized to the level of MEK phosphorylation in the absence of Plk1 (n = 4, *P < 0.05)

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