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Fig. 4 | Respiratory Research

Fig. 4

From: Increased ectodomain shedding of cell adhesion molecule 1 as a cause of type II alveolar epithelial cell apoptosis in patients with idiopathic interstitial pneumonia

Fig. 4

Increased apoptosis of NCI-H441 cells transfected with an siRNA targeting CADM1. a NCI-H441 cells were left untreated (–) or transfected with control or CADM1-targeting siRNA (siRNA-1 and -2). After 48 h, CADM1 expression was examined by Western blot analyses. The blot was reprobed using an anti-G3PDH antibody to normalize full-length CADM1 levels to G3PDH. After Western blot transfer, the gels were stained with CBB to indicate the amount of protein loading per lane. b The relative CADM1 levels were further normalized to a value of 1 in untreated cells, to which the CADM1 levels in transfectants were then normalized. The mean ± SE full-length CADM1 levels were calculated from triplicate experiments for each cell type, and statistical significance was analyzed using Student’s t-test. P-values ≤ 0.05 are shown. c Another set of NCI-H441 cells untreated and transfected with either control or siRNA-1 vector was analyzed by TUNEL assays. In the upper column, TUNEL (green) and DAPI (blue) fluorescent images were merged. The differential interference contrast images are shown on the lower. Bar = 50 μm. The mean proportions of TUNEL-positive cells and the SE were indicated under the panels. P-values ≤ 0.05 by Student’s t-test are shown. Results of siRNA-2 transfectants are shown in Additional file 6: Figure S5

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