Fig. 2
Correlation between AECII apoptosis and CADM1 ectodomain shedding in IIP. a Control and IIP lung sections were stained immunohistochemically with an anti-ssDNA antibody and counterstained with hematoxylin. A representative photomicrograph for each group is shown. Arrows indicate AECIIs detached from the alveolar wall. Arrowheads indicate ssDNA-positive inflammatory blood cells, as opposed to AECIIs. Bar = 50 μm. b IIP lung sections were double immunostained with antibodies against ssDNA (green) and SP-A (red), and counterstained with DAPI (blue). A representative result for each subtype is shown as a picture where the three fluorescence images are merged. Arrows indicate cells double positive for ssDNA and SP-A. Bar = 25 μm. c Proportions of ssDNA-positive AECIIs in each patient sample are plotted as dots, and statistical significance between two groups was analyzed using the Steel–Dwass test. P-values ≤ 0.05 are shown. d The proportion of ssDNA-positive AECIIs with the CADM1 α-shedding rate (left) and the full-length CADM1 level per epithelial cell (right) are shown in a scatter plot. In each graph, the dot distribution approximates a linear function (dotted lines). Correlations and statistical significance were analyzed using Spearman’s rank test. R 2 and P-values are shown