Fig. 6

Modulation of H3K18ac, ΔNp63, EGFR, and STAT6 expression with TSA treatment. a and b: 16HBE14o- cells were treated with TSA at 0, 10, 100, 500, and 1000 ng/μl for 24 h. Protein was collected and SDS-PAGE immunoblot was performed to quantify H3K18ac and total H3. Data are presented as fold change H3K18ac normalized to H3 ± SEM (n = 3). A one-way ANOVA and a post hoc Dunnett’s multiple comparison test was performed. c and d: AECs from healthy subjects were treated with TSA (100 ng/μl) for 24 h. Protein was collected and SDS-PAGE immunoblot was performed to quantify the change in ΔNp63, EGFR, and STAT6 protein between control and TSA treated samples. Data are presented as fold change normalized to loading control (β-tubulin for ΔNp63 and STAT6, and hsp90 for EGFR) ± SEM (n = 5). A two-tailed paired t-test was performed for control versus treatment for each gene. *indicates p < 0.05