Fig. 7
From: Increased FGF1-FGFRc expression in idiopathic pulmonary fibrosis
Impact of exogenous FGF1 on cell migration of IPF vs. Donor fibroblasts. Primary lung fibroblasts were starved for 24 h and seeded (12,000 cells/well) in the upper chamber of the transwell (6.5-mm transwell inserts with 8.0-μm pore size). Untreated (group 1) PD173074 (0.1 μM), (group 2), recombinant human FGF1 + heparin (25 ng/mL each) (group 3) or FGF1 + heparin + PD173074 (group 4) was added to the lower wells. Cells migrated for 16 h, were fixed and stained with crystal violet (a). The fold change of the number of migrated donor fibroblasts (b) and IPF fibroblasts (c). In both cases, PD173074 alone had no effect, FGF1 + heparin stimulated migration and and migration was attenuated in the FGF1 + heparin + PD173074 groups. Additional control experiments are available in Additional file 7: Figure S6. Graphs represent MetaMorph analyses of fibroblasts cultures for 18 h; blue = donor, red = IPF; total distance traveled in 18 h (d). FGF1 + heparin stimulated IPF fibroblasts to travel longer distances while no effect was observed in non-IPF, donor fibroblasts. Experiments were repeated in triplicate. Scale bars: (100 μm)