Fig. 1

Western blot and qPCR analyses of IPF and donor whole lung homogenate lysates and RNA revealed increased FGF1-FGFR expression. Western blots were performed on end-stage IPF (IPF) and non-IPF, donor lung homogenate lysates for FGF, MAPK and PI3/K signaling markers (a). Densitometry plots of arbitrary units indicated a significant increase in FGF1 (b), no increase in FGF7 (c), FGF10 (d). Receptors FGFR1 (e), FGFR2 (f), FGFR3 (g) but not FGFR4 (h) were increased in IPF samples as well as p-ERK1 (i), p-ERK2 (i’) and p-AKT (j). ACTA2 and COL1A1 transcripts were increased in IPF samples (k) as well as FGFR2b ligand transcripts: FGF1, FGF7, (l) and FGF10 (m). B-isoforms of FGFRs were decreased (n) while FGFR2 c-isoform was significantly increased (o). Expression of FGFR3 was variable and FGFR4 (p) was not changed between IPF and donor