Fig. 1
From: Hyperoxia-induced methylation decreases RUNX3 in a newborn rat model of bronchopulmonary dysplasia
RUNX3 expression in lung tissues and AT2 cells following hyperoxia. RUNX3 protein localization in lung tissues by immunohistochemistry showing RUNX3 protein expression in both the nucleus and cytoplasm of alveolar epithelial cells in control (a) and model groups (b). The arrow indicates positive cells. (×400). Western blot of RUNX3 protein (c and d), and real-time PCR of RUNX3 mRNA (e) showing low expression in lung tissues of the model group after 7 d hyperoxia. Low expression of RUNX3 protein (f and g) and mRNA (h) in AT2 cells from the model group after 7 d hyperoxia. C: Control group, E: model group, *P < 0.05 vs. control group, n = 4