Fig. 2
The formation of advanced glycation end products (AGEs) increased with collagen glycation. AGEs formation was evaluated in non-cellular post-glycated DMEM (a1-3) and PBS (b1-3) matrices based on autofluorescence (ex/em: 360/440 nm wavelengths). The matrices were elaborated using different concentrations of fetal bovine serum (FBS), 0 % (a1, b1), 1 % (a2, b2) and 10 % (a3, b3). The media was changed every 2 days beginning on the 5th day to allow collagen cross-linking to occur. AGE formation in both type of matrices (DMEM and PBS), was significantly greater with higher ribose concentrations (240 mM) (p < 0.01) and at the 7th day (p < 0.01). After that, less autofluorescence was observed for all the matrices suggesting less AGEs’ formation. Interestingly, DMEM matrices (2A) were more autofluorescent than PBS matrices (2B) at days 1 and 7 (p < 0.05 in practically all conditions). Nevertheless, PBS matrices became more autofluorescent than DMEM ones after the 14th day (p < 0.05 in practically all conditions for FBS 1 % and 10 %). The experiments were repeated three times with similar results obtained. * p < 0.05