Fig. 3

6-MP inhibits activation of the NFκB pathway. a Serum-starved NCI-H292 cells were pre-treated with 6-MP and then stimulated with TNFα for the indicated time periods. Western blot analysis for pIkBα was performed and β-actin was used as a loading control. b NCI-H292 cells were transfected with a NFκB-reporter plasmid and TNFα-induced luciferase activity was measured in the absence and in the presence of 6-MP. c The NFκB subunit p65 was overexpressed together with the NFκB-reporter plasmid and luciferase activity was measured after 36 h. The transfection efficiencies were normalized using Renilla luciferase co-transfection. d Rac1 activity is decreased by 6-MP in MLE-12 cells. Values represent mean ± S.D. *, p ≤ 0.05. a.u = arbitrary units