Fig. 5

Loss of LMO4 does not alter overall survival in mouse model of lung cancer. a Kaplan-Meier curve showing overall survival of K-Ras ^LSL-G12D/+ ;Lmo4 ^fl/fl, K-Ras ^LSL-G12D/+ ;Lmo4 ^fl/+ and K-Ras ^LSL-G12D/+ ;Lmo4 ^+/+ mice following cre recombination with Ad5-CMV-cre adminstration. b Representative H&E showing overall tumour burden in K-Ras ^LSL-G12D/+ ;Lmo4 ^fl/fl and K-Ras ^LSL-G12D/+ ;Lmo4 ^+/+ mice. Scale bar = 2mm. c Average lung weight of K-Ras ^LSL-G12D/+ ;Lmo4 ^fl/fl, K-Ras ^LSL-G12D/+ ;Lmo4 ^fl/+ and K-Ras ^LSL-G12D/+ mice at ethical endpoint collection indicating overall tumor burden. The graph represents mean ± S.E.M., n ≥ 13 animals per group. d Immunohistochemistry showing the expression of TTF-1 and Sox2 in K-Ras ^LSL-G12D/+ ;Lmo4 ^fl/fl and K-Ras ^LSL-G12D/+ ;Lmo4 ^+/+ tumours. Scale bars = 50μm. e Representative H&E staining of K-Ras ^LSL-G12D/+ ;Lmo4 ^fl/fl and K-Ras ^LSL-G12D/+ ;Lmo4 ^+/+ hyperplastic airways 12 weeks following intranasal administration of Ad5-CMV-cre. Scale bars = 100μm. f Quantification of the number of BrdU⁺ cells in hyperplastic airways of K-Ras ^LSL-G12D/+ ;Lmo4 ^fl/fl and K-Ras ^LSL-G12D/+ mice at 12 weeks post Ad5-CMV-cre administration. Data represent number of BrdU⁺ cells per unit of airway area. The graph represents mean ± S.E.M., n ≥ 18 hyperplastic airways per group (n ≥ 3 animals per group)