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Fig. 4 | Respiratory Research

Fig. 4

From: The LIM-domain only protein 4 contributes to lung epithelial cell proliferation but is not essential for tumor progression

Fig. 4

Loss of Lmo4 affects early stages of K-RasG12D-induced cell proliferation. a H&E and BrdU staining of K-Ras LSL-G12D/+ ;Lmo4 fl/fl and K-Ras LSL-G12D/+ Lmo4 +/+ lungs six days following naphthalene injection. Scale bars = 100μm. Dashed line shows region used for quantification of BrdU staining. b Quantification of the number of BrdU+ cells per unit of airway area in K-Ras LSL-G12D/+ ;Lmo4 fl/fl, K-Ras LSL-G12D/+ ;Lmo4 fl/+ and K-Ras LSL-G12D/+ ;Lmo4 +/+ mice six days following naphthalene injection. n ≥ 3 animals per group. c H&E and BrdU staining of K-Ras LSL-G12D/+ ;Lmo4 fl/fl and K-Ras LSL-G12D/+ Lmo4 +/+ hyperplastic airways 11 weeks post naphthalene administration. Mice received Ad5-CMV-cre one week prior to naphthalene treatment. Scale bars = 100μm. d Quantification of the number of BrdU+ cells in K-Ras LSL-G12D/+ ;Lmo4 fl/fl and K-Ras LSL-G12D/+ ;Lmo4 +/+ mice at 11 weeks post naphthalene administration. Mice received Ad5-CMV-cre one week prior to naphthalene treatment. Data represent number of BrdU+ cells per unit of airway area. The graph represents mean ± S.E.M., n ≥ 26 hyperplastic airways per group (n ≥ 3 animals per group)

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