Fig. 5

Increased Col1 and Col3 deposition, and Col1:Col3 ratio differences, in UIP or COP versus healthy lung. a Compared to healthy, Col1 deposition was significantly increased in UIP (**p < .0033) and trended toward an increase in COP (p = .13). b Col3 deposition was about equally increased in both COP (**p < .009) and UIP (**p < .004) versus healthy. Overall, this led to c The Col1:Col3 ratio being effectively equivalent in COP versus healthy, but significantly increased in UIP versus healthy (*p < .015). Plots were generated from ≥ 9-18 sections and ≥ 45–90 distinct ROIs from N ≥ 3 patients per condition. All data was plotted as N = number of patients per group. Statistics were performed by one way ANOVA with Dunnett’s post-hoc test and correction for multiple comparisons against healthy control. Values for Collagen I and III represent mean immunofluorescence pixel intensities in relative AFU (Panels a, b), or a ratio thereof (Panel c). In panels d–f, for illustrative purposes, the originally grayscale Col3 immunofluorescence is shown with “Red” LUT applied in ImageJ for Healthy, COP, and UIP respectively, with levels (screen stretch) linear and set the same for all images. Note the round Masson’s bodies characteristic of COP (in e), whereas f respresents an area of widespread late-stage fibrosis in UIP