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Fig. 4 | Respiratory Research

Fig. 4

From: Second harmonic generation microscopy reveals altered collagen microstructure in usual interstitial pneumonia versus healthy lung

Fig. 4

Lung tissue with preserved alveolar architecture adjacent to UIP fibroblastic foci and honeycomb areas have different fibrillar collagen microstructure versus healthy lung. SHG imaging of the FSHG/BSHG ratio was performed on healthy (Hlthy) and UIP lung tissue in areas with preserved alveolar architecture adjacent (UIPadj) and compared to UIP fibroblastic foci (UIPff) and honeycomb regions (UIPhc). Methodology was otherwise as described in Fig. 1. The FSHG/BSHG ratio was significantly decreased in UIPadj (**p < .0036) and UIPff (**p < .0054) and UIPhc (**p < .0007) versus healthy lung tissue – whereas UIPadj, UIPff, and UIPhc were not significantly different from each other – suggesting that even “normal appearing” lung tissue in UIP patients has altered FC microstructure. Plots were generated from a total of 15–30 sections and 75–150 distinct ROIs from N = 5 patients (Healthy and UIPff); 18–36 sections and 90–180 distinct ROIs from N = 6 patients (UIPadj); and 30–60 sections and 150–300 distinct ROIs from N = 10 patients (UIPhc). All data was plotted as N = number of patients per group. Statistics were performed by one way ANOVA with Dunnett’s post-hoc test and correction for multiple comparisons against healthy control. F/B SHG values are a ratio of mean pixel intensities in relative AFU

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