Fig. 3
Healthy, COP, and UIP lung histopathology compared to FSHG. Representative hematoxylin and eosin (H&E) FOVs showing healthy a versus fibrotic COP b and UIP c pathology were field-matched to the FSHG images d, e, f for the same FOVs, respectively. Note the eosin stained areas of concentrated collagen deposition (light pink color, indicated by blue arrows in b and c) that match areas of high FC FSHG signal intensity (white pixel regions, indicated by blue arrows in e and f) in COP and UIP respectively. In contrast, the FSHG (collagen) signal in healthy tissue d arises primarily from alveolar parenchyma and muscular arteries (examples of muscular arteries are indicated by yellow arrows, in all images). Thus SHGM detects and allows quantification of altered microstructure (e.g. Figs. 1, 3 and Fig. 5) from both intrinsic normal and pathologic collagen content in lung tissue. Levels (screen stretch) are linear and set the same for all images d–f