Fig. 1
Fibrillar collagen microstructure is different in UIP, but not COP, versus healthy lung. SHG imaging was performed on healthy, COP, and UIP formalin fixed paraffin embedded human lung tissue, and the FSHG/BSHG ratio was calculated to assess relative differences in FC microstructure. Plot represents mean FSHG/BSHG pixel intensity ± SEM for each disease, calculated as described in Methods. Compared to healthy lung tissue, FSHG/BSHG was significantly decreased only in UIP (**p < .0018) but not COP. Plots were generated from a total of 15–30 sections and 75–150 distinct ROIs from N = 5 patients (Healthy and COP), and 30–60 sections and 150–300 distinct ROIs from N = 10 patients (UIP). All data was plotted as N = number of patients per group. Statistics were performed by one way ANOVA with Dunnett’s post-hoc test and correction for multiple comparisons against healthy control. F/B SHG values are a ratio of mean pixel intensities in relative arbitrary fluorescent units (AFU)