Fig. 4
The effects of c-Src inhibitor PP2 and RhoA inhibitor Y27632 on epithelia gap formation. MLE-12 cells were pretreated with PP2 (100 μmol/L) or Y27632 (20 μM) for 30 min before cyclic stretching. (a, b) The effects of PP2 or Y27632 on the activation of c-Src or RhoA. (c) The expression of adherens junction and tight junction proteins after PP2 or Y27632 treatment. (d) The epithelial gap formation determined by immunofluorescence and quantification data for the gap formation. Scale bars = 10 μm. *p < 0.05 versus control group, +p < 0.05 versus CS group, #p < 0.05 versus DMSO + CS group. Experiments were repeated at least three times