Fig. 3

The relation between p120 and JMD domain contributed to the endocytosis of E-cadherin. MLE-12 cells were transfected with WT-JMD or flag-K83R-JMD using lipofectamine 2000. (a) Mouse E-cadherin JMD sequence and the mutant JMD sequence. (b) Successful transfection of flag-K83R-JMD was determined by western blot. (c) The association of p120 and E-cadherin in the JMD mutant transfection group. K83R mutant abolished E-cadherin JMD/p120 binding. (d) MLE-12 cells were transfected with WT-JMD or K83R-JMD. And the E-cadherin JMD/120 binding was determined by immunofluorescence. The nucleus were stained with DAPI (blue). Scale bars = 10 μm. *p < 0.05 versus control group, +p < 0.05 versus CS group. Experiments were repeated at least three times