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Figure 4 | Respiratory Research

Figure 4

From: Decreased expression of the NF-κB family member RelB in lung fibroblasts from Smokers with and without COPD potentiates cigarette smoke-induced COX-2 expression

Figure 4

Cox-2 mRNA and protein expression is induced by 2% CSE in Smoker and COPD lung fibroblasts. (A) Cox-2 mRNA- CSE: There was a significant increase in Cox-2 mRNA in COPD lung fibroblasts (black bars) exposed to 2% CSE for 3 hours (*** p <0.05 compared to respective control); there was a significantly more Cox-2 mRNA in COPD fibroblasts at 6 and 24 hours of exposure. There was a trend towards increased Cox-2 mRNA in At Risk fibroblasts (grey bars). Note the lack of relative Cox-2 mRNA induction in Normal lung fibroblasts (white bars). Results are expressed as the mean ± SEM of 4–9 fibroblasts from each subject group. (B) COX-2 protein- CSE: There was a modest induction in COX-2 protein in Normal fibroblasts. There was noticeably more COX-2 protein in the At Risk and COPD fibroblasts. The induction in COX-2 protein occurred earlier COPD fibroblasts (by 3 hours). Representative western blot is shown of at least 3 different experiments.

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