Figure 4

Suppressive effects of astragalin on collagen type-1 production in H₂O₂-exposed BEAS-2B cells (A) and collagen fiber deposition in OVA-challenged mouse airways (B). BEAS-2B cells were cultured with 20 μM H₂O₂ in the absence and presence of 1–20 μM astragalin for 72 h. Culture media were prepared for Western blotting with a primary antibody against collagen type-1 (A). The bar graphs (mean ± SEM, n = 3) represent quantitative results of the upper bands obtained from a densitometer. Means without a common letter differ, P < 0.05. Pathological collagen fiber deposition in mouse airways was observed by Masson trichrome staining (B). The collagen fibers were stained in blue and quantified (bottom bar graphs, mean ± SEM). Each photograph is representative of four mice. Bar = 100 μm.