Figure 2

Temporal response of E-cadherin loss (A), and inhibitory effects of astragalin on E-cadherin loss (B) and vimentin induction (C). After cells were treated with 1–20 μM astragalin and stimulated with 20 μM H₂O₂ for up to 72 h, cell lysates were prepared for Western blotting with a primary antibody against E-cadherin and vimentin. β-Actin protein was used as an internal control. The bar graphs (mean ± SEM, n = 3) represent quantitative results of the upper bands obtained from a densitometer. Means without a common letter differ, P < 0.05.