Figure 2
Dynamics of TGFβ and BMP signaling of wild-type mice in bleomycin-induced lung fibrosis. (A) Gene expression level of TGFβ 1 , Collagen1α2, Bmp2, Bmp4, Bmp6 and Bmp7 (n = 5 at day 0, n = 4 at day 7 and 14, n = 2 at day 21, data are mean and SEM of ΔΔCT values normalized to control conditions and Hprt). (B) Gene expression level of noggin (n = 3 at day 0 and day 1, n = 4 at day 7 and 14, n = 2 at day 21, data are mean and SEM of ΔΔCT values normalized to control conditions and Hprt). (C) Western blot of representative lung protein extracts from mice 1, 3, 7, 14 and 21 days after bleomycin instillation, labeled with antibodies against pSMAD3, pSMAD1/5/8 and GAPDH.