Figure 8

Antioxidant N-acetylcysteine (NAC) prevents the inhibition of virus-mediated BAFF induction by cigarette smoke extract (CSE). (A) BEAS-2B cells were treated with indicated concentrations of CSE and/or infected with influenza virus at a multiplicity of infection (MOI) of 0.5 for 24 hours. (B) BEAS-2B cells were treated with indicated concentrations of interferon beta (IFN-β) for 24 hours. (C) BEAS-2B cells were treated with indicated concentrations of IFN-β in the combination with 5% CSE and influenza virus at 0.5 MOI for 24 hours. (D) BEAS-2B cells were pretreated with indicated concentrations of N-acetylcysteine (NAC) for 2 hours and then treated with 5% CSE and influenza virus at 0.5 MOI for 24 hours. The relative mRNA levels of BAFF in BEAS-2B cells are shown. CTRL, without influenza virus infection; Flu, with influenza virus infection; Flu + CSE, with CSE treatment and influenza virus infection; NS, nonsignificant; *P < 0.05; **P < 0.01.