Figure 5

Cigarette smoke (CS) exposure prior to influenza virus infection results in reduced BAFF expression. Mice were exposed to room air (RA) or CS for 3 months and then infected with influenza virus or vehicle control. They were sacrificed and evaluated on day 7 after infection. Representative immunohistofluorescence stained lung sections from (A) RA-exposed mice with influenza virus infection (cells in bronchial lumen), (B) CS-exposed mice with influenza virus infection (cells in bronchial lumen), (C) RA-exposed mice with influenza virus infection (bronchial epithelium) and (D) CS-exposed mice with influenza virus infection (bronchial epithelium) are shown (BAFF: red, nuclear stain DAPI: blue). Original magnification, ×200.