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Figure 9 | Respiratory Research

Figure 9

From: IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model

Figure 9

IL-25, but not IL-4, IL-5 or IL-13 induced angiogenesis in vitro. Top panel: representative light photomicrographs (4x original magnification) show formation of primitive vascular tubule structures by human vascular endothelial cells after 11 days of culture (top panel) with medium (A), VEGF (10 ng/mL) (B), IL-25 (10 ng/mL) (C), IL-4 (10 ng/mL) (D), IL-5 (10 ng/mL) (E) and IL-13 (10 ng/mL) (F). Bottom panel: computer-assisted quantification of total tubule lengths (G), numbers of branch points (H) and total numbers of tubules (I). Bars show the mean ± SEM of three separate experiments performed in duplicate. *p < 0.05. Mann–Whitney U test. *p < 0.05.

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