Figure 7
From: Tight junction disruption by cadmium in an in vitro human airway tissue model
Protective effects of kinase inhibitors for c-Src and PKC on Cd-induced TJ disruption. (A). TJ integrity was assessed using immunofluorescence staining of ZO-1 and occludin. Cotreatment of CdCl2 and kinase inhibitors prevented Cd-induced TJ disruption. Descriptions of the individual lettered panels are given in the text. (B). Representative Western blots showing protein expression of cingulin, TJAP1, and VAP-33. Kinase inhibitors failed to prevent the down-regulation of these junction-interacting proteins. (C). Density of the Western blots in Figure 7B. were quantified and statistically analyzed (N = 3). *Indicates p < 0.05 compared to the vehicle treated control. (D). Tyr-phosphorylation of occludin was modulated by CdCl2. Cultures were treated from the basolateral side with 100 μM CdCl2 in the presence or the absence of kinase inhibitors for c-Src or PKC. Tyrosine phosphorylated occludin was detected in occludin-enriched immunoprecipitates. Both kinase inhibitors prevented Tyr hyperphosphorylation of occludin.